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primary antibodies against chop  (Cell Signaling Technology Inc)
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RNA isolation and quantitative real-time PCR assay
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Gene primer sequences.

Journal: Frontiers in Pharmacology

Article Title: Licorice attenuates cisplatin-induced hepatotoxicity by alleviating endoplasmic reticulum stress and apoptosis

doi: 10.3389/fphar.2025.1557125

Figure Lengend Snippet: Gene primer sequences.

Article Snippet: WB analysis was performed as previously described ( ) using specific primary antibodies against CHOP (1:1000, 15204-1-AP, Proteintech), ATF4 (1:1000, BM5179, Boster), ATF6 (1:1000, A00655, Boster), GRP78 (1:1000, BA 2042, Boster), p-IRE1α (1:1000, NB100-2323, Novus), p-eIF2α (1:1000, BM3942, Boster), Bcl-2 (1:1000, A00040-1, Boster), Bax (1:1000, A00183, Boster), cleaved caspase-3 (1:1000, #9664T, Cell Signaling Technology), caspase-12 (1:1000, BA3142, Boster), cleaved caspase-8 (1:5000, ab108333, Abcam), p-PERK (1:1000, abs137056, Absin) and β-actin (1:5000, 20536-1-AP, Proteintech).

Techniques:

GC attenuated CP-induced apoptosis by modulating the ER-mediated PERK/ATF4/CHOP pathway. (A) The expressions of p-PERK, p-eIF2α, ATF4 and CHOP proteins were detected by WB analysis. (B) qRT-PCR was performed to detect the expression of CHOP and ATF4 mRNA. (C) Representative image and quantification of the immunofluorescence of CHOP and ATF4. (D) The expressions of cleaved caspase-3, cleaved caspase-8, and caspase-12 proteins were detected by WB analysis. (E) qRT-PCR was performed to detect the expression of caspase-8, and caspase-12 mRNA. All data are presented as the mean ± SD. ## p < 0.01 compared with control group; * p < 0.05, ** p < 0.01 compared with CP group.

Journal: Frontiers in Pharmacology

Article Title: Licorice attenuates cisplatin-induced hepatotoxicity by alleviating endoplasmic reticulum stress and apoptosis

doi: 10.3389/fphar.2025.1557125

Figure Lengend Snippet: GC attenuated CP-induced apoptosis by modulating the ER-mediated PERK/ATF4/CHOP pathway. (A) The expressions of p-PERK, p-eIF2α, ATF4 and CHOP proteins were detected by WB analysis. (B) qRT-PCR was performed to detect the expression of CHOP and ATF4 mRNA. (C) Representative image and quantification of the immunofluorescence of CHOP and ATF4. (D) The expressions of cleaved caspase-3, cleaved caspase-8, and caspase-12 proteins were detected by WB analysis. (E) qRT-PCR was performed to detect the expression of caspase-8, and caspase-12 mRNA. All data are presented as the mean ± SD. ## p < 0.01 compared with control group; * p < 0.05, ** p < 0.01 compared with CP group.

Article Snippet: WB analysis was performed as previously described ( ) using specific primary antibodies against CHOP (1:1000, 15204-1-AP, Proteintech), ATF4 (1:1000, BM5179, Boster), ATF6 (1:1000, A00655, Boster), GRP78 (1:1000, BA 2042, Boster), p-IRE1α (1:1000, NB100-2323, Novus), p-eIF2α (1:1000, BM3942, Boster), Bcl-2 (1:1000, A00040-1, Boster), Bax (1:1000, A00183, Boster), cleaved caspase-3 (1:1000, #9664T, Cell Signaling Technology), caspase-12 (1:1000, BA3142, Boster), cleaved caspase-8 (1:5000, ab108333, Abcam), p-PERK (1:1000, abs137056, Absin) and β-actin (1:5000, 20536-1-AP, Proteintech).

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Control

RNA isolation and quantitative real-time PCR assay

Journal: Archives of toxicology

Article Title: Study of the roles of cytochrome P450 (CYPs) in the metabolism and cytotoxicity of perhexiline

doi: 10.1007/s00204-022-03369-0

Figure Lengend Snippet: RNA isolation and quantitative real-time PCR assay

Article Snippet: Primary antibodies against CHOP (Cat# 2895, Cell Signaling Technology, Danvers, Massachusetts) and GAPDH (Cat# sc-47724, Santa Cruz Biotechnology, Santa Cruz, CA) were used.

Techniques: Isolation, Real-time Polymerase Chain Reaction